RNAplant
植物(wù)RNA提取試劑
産品簡介：
RNAplant試劑可從(cóng)植物(wù)組織，特别是富含多(duō)酚或澱粉的植物(wù)組織中（如馬鈴薯塊莖，白(bái)松松針或嫩苗，和西(xī)紅(hóng)柿葉等）提取高(gāo)純度的總RNA。
每100 ml可處理100 mg組織200次。
保存條件(jiàn)：室溫運輸，4℃保存，可保存6-12個(gè)月(yuè)。
準備試劑：液氮，研缽，無RNase離心管，5 M NaCl（NC1050F 5M 氯化鈉 RNase-free，DNase-free），氯仿，異丙醇，75%乙醇（無RNase水(shuǐ)配制），無RNase水(shuǐ)。
小(xiǎo)規模提取操作步驟：（樣品<0.1 g ，使用小(xiǎo)規模提取方法）：
1. 取不多(duō)于0.1 g植物(wù)組織，在液氮中研碎，轉移到(dào)1.5 ml 離心管中，加0.5 ml提取試劑，振蕩至徹底混勻。
注：振蕩不要太劇烈，否則容易導緻基因組DNA污染。
2. 室溫放(fàng)置5分鍾。
3. 4℃ 12, 000 rpm離心2-3分鍾，上(shàng)清轉入新的無RNase離心管。
4. 上(shàng)清中加入0.1 ml 5 M NaCl（NC1050F 5M 氯化鈉 RNase-free，DNase-free），溫和混勻。
5. 加入0.3 ml 氯仿，上(shàng)下(xià)颠倒混勻。
6. 4℃ 12,000 rpm離心10-15分鍾，取上(shàng)層水(shuǐ)相(xiàng)轉入新的無RNase離心管。
7. 加與所得水(shuǐ)相(xiàng)等體積的異丙醇，混勻，室溫放(fàng)置10分鍾。
8. 4℃ 12,000 rpm離心10-15分鍾。棄掉上(shàng)清，注意不要倒出沉澱。加1 ml 75%乙醇沖洗沉澱。（沉澱可能(néng)很難看(kàn)見(jiàn)，應小(xiǎo)心操作。）
9. 4℃ 5000-7000rpm離心3分鍾。倒出液體，注意不要倒出沉澱。剩餘的少量液體短暫離心，用槍頭吸出，室溫晾幹1-2分鍾至沉澱幹燥。
10. 加适量無RNase水(shuǐ)充分溶解RNA，-70℃保存。
注意事(shì)項：
RNAplant有刺激性味道，操作時請在通(tōng)風處進行。
RTR2303 RNAplant 發表文章列表：
1. [2021
IF=4.7] The 5-untranslated region of the FAD3 mRNA is required for its
translational enhancement at low temperature in Arabidopsis roots.
Author: Chun-TaoWang, Yi-Nong Xu.
Journal: Plant Science 179,234-240,2010
植物(wù)材料：拟南(nán)芥
Institution: Key Laboratory of Photobiology, Institute of
Botany, Chinese Academy of Sciences,
Paper
link：https://linkinghub.elsevier.com/retrieve/pii/S0168945210001470
2. [2021
IF=4.1] Different exogenous sugars affect the hormone signal pathway and
sugar metabolism in ‘‘Red Globe’’ (Vitis vinifera L.) plantlets grown in vitro as shown by transcriptomic
analysis.
Author: Juan Mao,Wenfang Li,Baoqin Mi,Zonghuan Ma,Yongmei Zhang, Baihong
Chen
Journal: Planta 246,
537–552,2017
植物(wù)材料：葡萄
Institution: College of Horticulture, Gansu Agricultural
University
Paper
link：https://link.springer.com/article/10.1007/s00425-017-2712-x
3. [2021
IF=3.4] Transcriptome analysis revealed glucose application afects plant
hormone signal transduction pathway in “Red Globe” grape plantlets s.
Author: Juan Mao ,Wenfang Li, Baoqin Mi, Zonghuan Ma, Mohammed
Mujitaba Dawuda, Cunwu Zuo
Journal: Plant Growth Regul volume 84, pages 45–56 (2018)
植物(wù)材料：葡萄
Institution: College of Horticulture, Gansu Agricultural University
Paper
link：https://link.springer.com/article/10.1007/s10725-017-0320-1
4. [2021
IF=4.169] Effects of CEPA and 1-MCP on flower bud differentiation of apple
cv. 1 agafu No.2 grafted on different rootstocks.
Author: Wenfang Li, Baihong Chen
Journal: J Plant Growth Regul. volume 38, pages 842–854 (2019)
植物(wù)材料：蘋果
Institution: College of Horticulture, Gansu Agricultural
University
Paper
link：https://link.springer.com/article/10.1007/s00344-018-9895-7
5. [2021
IF=7.228] FIT and bHLH Ib transcription factors modulate iron and copper
crosstalk in Arabidopsis.
Author: Yuerong Cai, Yang Li, Gang Liang
Journal: Plant, Cell and Environment. Volume44,
Issue5 May 2021 Pages 1679-1691
植物(wù)材料：拟南(nán)芥
Institution: CAS Key Laboratory of Tropical Plant Resources and
Sustainable Use, Xishuangbanna Tropical Botanical Garden
Paper
link：https://onlinelibrary.wiley.com/doi/10.1111/pce.14000
6. [2022
IF=6.2] Genome-Wide Identification and Abiotic Stress Response Analysis
of PP2C Gene Family in Woodland and Pineapple Strawberries.
Author: Lili Guo, Shixiong Lu, Tao Liu, Guojie Nai, Jiaxuan Ren, Huimin
Gou, Baihong Chen and Juan Mao
Journal: International Journal
of Molecular Sciences. 2023, 24, 4049.
植物(wù)材料：草(cǎo)莓
Institution: College of Horticulture, Gansu Agricultural
University
Paper
link：https://doi.org/10.3390/ijms24044049
7. [2022
IF=2.561] Physiological and Transcriptomic Analysis Reveals Drought?Stress Responses
of Arabidopsis DREB1A in Transgenic Potato
Author: Xiaoxia Jia, Enfang Qi, Shi Liu, Sheng Ma, Guohong Wen,
Xinhui Zhang, Heping Lv, Wei Huang, Xucheng Zhang
Journal: Potato Research Published online 21 Feb 2023
植物(wù)材料：馬鈴薯
Institution: Gansu Academy of Agricultural Sciences
Paper
link：https://link.springer.com/article/10.1007/s11540-023-09619-7
8. [2022
IF=6.6] Molecular evolution and expression assessment of DFRs
in apple
Author: Wen?Fang Li, Ju Gao, Zong?Huan Ma, Ying?Jun Hou, Xin Li, Juan Mao and Bai?Hong Chen
Journal: Chemical and
Biological Technologies in Agriculture (2023) 10:98
植物(wù)材料：蘋果
Institution: College of Horticulture, Gansu Agricultural
University
Paper
link： https://doi.org/10.1186/s40538-023-00470-z
9. [2022
IF=3.5] Genome-wide identification and expression analysis of the BURP
domain-containing genes in Gossypium hirsutum
Author: Jiaxuan Ren,Li Feng,Lili Guo,
Huimin Gou,Shixiong Lu, Juan Mao
Journal: Physiology and
Molecular Biology of Plants 2023
29(11):1717–1731
植物(wù)材料：棉花
Institution: College of Horticulture, Gansu Agricultural
University
Paper
link：https://doi.org/10.1007/s12298-023-01393-7