D2000 DNA ladder（100-2000bp）

本産品是由6條帶狀雙鏈DNA條帶組成，适用于瓊脂糖凝膠電(diàn)泳中DNA條帶的分析。産品中已含有1×loading buffer，可根據實驗需要，直接取2-5μl電(diàn)泳，使用方便，電(diàn)泳圖像清晰。6條帶的大小(xiǎo)分别為(wèi)100，250，500，750，1000，2000bp。其中750bp條帶為(wèi)100ng/5μl，顯示加亮帶，其餘條帶濃度50ng/5μl。

● 使用方法：

1. 取2-5μl本産品加入到(dào)瓊脂糖凝膠的加樣孔中（每1mm×1mm加樣孔加1μl，如果加樣孔寬于

5mm，可以适當增加上(shàng)樣量）就(jiù)行電(diàn)泳。

2. 建議電(diàn)泳條件(jiàn)：凝膠濃度為(wèi)1.5-2.0％，凝膠長(cháng)度5-7cm，電(diàn)泳電(diàn)壓4-10v/cm，電(diàn)泳時間15-20

分鍾。

3. 通(tōng)過EB染色後紫外燈下(xià)觀察條帶。

注：如果使用Goldview等染料就(jiù)行染色，由于靈敏度比EB低(dī)，請酌情增加Marker的上(shàng)樣量。

● 注意事(shì)項：

1. 瓊脂糖的質量對DNA的電(diàn)泳有很大影響，電(diàn)泳時請盡量使用質量優等的瓊脂糖。

2. 請使用新鮮配制的電(diàn)泳緩沖液和新鮮配制的瓊脂糖凝膠進行電(diàn)泳，以保證Marker良好的分離效果。

質檢證書下(xià)載

使用RTM415 D2000 DNA ladder産品發表部分文章列表：

1. [2021 IF=20.37] A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis)

Marker :RTM415，D2000 DNA ladder

Author: Dhanushka Udayanga & Xingzhong Liu &Pedro W. Crous & Eric H. C. McKenzie &Ekachai Chukeatirote & Kevin D. Hyde

Journal: Fungal Diversity (2012) 56:157–171

Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link：https://link.springer.com/article/10.1007/s13225-012-0190-9

2. [2021 IF=1.17] Rhopalostroma brevistipitatum sp. nov. from Thailand with an extended generic description for Rhopalostroma.

Marker :RTM415，D2000 DNA ladder

Author: DINUSHANI A. DARANAGAMA, XINGZHONG LIU, SUNITA CHAMYUANG, MARC STADLER，ALI H.BAHKALI, KEVIN D. HYDE

Journal: Phytotaxa，2015，227 (3): 229–242

Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link：https://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.227.3.2/0

3. [2021 IF=3.24] Envelope Proteins ofWhite Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT).

Marker :RTM415，D2000 DNA ladder

Author: Shijun Xie, Xiaojun Zhang, Jiquan Zhang, Fuhua Li, Jianhai Xiang

Journal: Plos One，Published: December 21, 2015

Institution：Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences

Paper link： https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144922

4. [2021 IF=2.88] Genetic diversity and genetic structure of Decalobanthus boisianus in Hainan Island, China.

Marker :RTM415，D2000 DNA ladder

Author: Huan Jiang，Wenxing Long， Hui Zhang，Chengneng Mi，Tao Zhou，Zongzhu Chen

Journal: Ecology and Evolotion Volume 569, 15 March 2019, Pages 28-30

Institution：Institute of Tropical Agriculture and Forestry, Hainan University

Paper link： https://doi.org/10.1002/ece3.5127

5. [2021 IF=1.17] Dendrostoma covidicola sp. nov. (Erythrogloeaceae, Diaporthales) on Fagus sylvatica from Sichuan Province, China.

Author: MILAN C. SAMARAKOON, ITTHAYAKORN PROMPUTTHA & JIAN-KUI (JACK) LI

Marker: RTM415，D2000 DNA ladder

Journal: Phytotaxa. 2021，483 (2): 085–094.

Institution：University of Electronic Science and Technology of China

Paper link：https://doi.org/10.11646/phytotaxa.483.2.1

6. [2021 IF=2.6] Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.

Marker :RTM415，D2000 DNA ladder

Author: Dongmei Jiang, Dizhe Wei, Hongtao Li, Liuqing Wang, Nan Jiang,Yunchao Li and Meng Wang

Journal: Journal of the Science of Food and Agriculture Volume101, Issue7，2021，3071-3077

Institution：Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences

Paper link：https://onlinelibrary.wiley.com/doi/10.1002/jsfa.10901

7. [2021 IF=20.37] Taxonomy, Phylogeny, Molecular Dating and Ancestral State Reconstruction of Xylariomycetidae (Sordariomycetes)

Marker :RTM415，D2000 DNA ladder

Author: Kevin D Hyde，Jian-Kui Liu

Journal: Fungal Diversity volume 112, pages 1–88 (2022)

Institution：School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China

Paper link：https://link.springer.com/article/10.1007/s13225-021-00495-5

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