Zeocin Selection Reagent

● 産品規格

Zeocin粉末型

● 産品說明

Zeocin? Reagent is a member of the bleomycin antibiotic family. Resistance is conferred by the Sh ble gene which encodes a 13,665 dalton protein. In cells expressing the protein, Zeocin? is prevented from binding and cleaving the cellular DNA. Zeocin? is effective on most aerobic cells and can be used for selection in mammalian and insect cell lines, yeast, and bacteria. The amount of Zeocin? required for selection will vary between 50-2000 μg/ml (typically 300μg /ml) depending on the cell type.

Zeocin可選擇Sh ble基因表達的細胞。與現在使用的其他動物(wù)細胞标記物(wù)無交叉抗性。因此這種抗生(shēng)素可用來分離對其他篩選劑（如：慶大黴素，潮黴素）有抗性的克隆。Zeocin是一(yī)種屬于争光(guāng)黴素家族的糖蛋白(bái)抗生(shēng)素，在體内能(néng)作用于大多(duō)數細菌（包括E. coli）、真菌（如：酵母菌）、植物(wù)細胞、動物(wù)細胞。

● 液體Zeocin可用于細胞培養。

● 用于篩選轉化株的Zeocin濃度根據pH值和鹽濃度改變。pH越高(gāo)，鹽濃度越低(dī)，Zeocin活性越大。

● 普通(tōng)大腸杆菌菌株篩選可在25 μg/ml zeocin濃度的低(dī)鹽LB瓊脂培養基（yeast extract 5 g/L, Tryptone 10 g/L, NaCl 5 g/L, Agar 15 g/L,pH 7.5）中進行，pH調至7.5 。

● 動物(wù)細胞篩選：根據不同細胞系從(cóng)100 到(dào) 750 μg/ml。

● 植物(wù)細胞篩選：根據不同的細胞種類和培養基從(cóng)50 到(dào) 250 μg/ml 。

● 酵母篩選：取決于種屬和培養基，S. cerevisiae Sh ble轉化株在50 μg/ml 濃度的YPD培養基中篩選，pH調至7.0。

● 粉末型配制方法：

取1 ml Zeocin溶解液加到(dào)Zeocin幹粉中，徹底溶解，用0.22 μm 一(yī)次性濾器(qì)抽濾除菌，即配成1 ml（100mg/ml）的Zeocin溶液，-20℃保存。

● 技(jì)術(shù)參數：

Empirical formula: C₅₅H₈₅O₂₁N₂₀S₂Cu·HCl

Molecular weight: 1525

CAS：11006-33-0